Pipet 1 ml of each dilution into separate, duplicate, appropriately marked petri dishes. Shake all dilutions 25 times in 30 cm (1 ft) arc within 7 s. Using separate sterile pipets, prepare decimal dilutions of 10 -2, 10 -3, 10 -4, and others as appropriate, of food homogenate ( see Chapter 1 for sample preparation) by transferring 10 ml of previous dilution to 90 ml of diluent.
#Parallel dilution calculator free
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